4 شهریور 1398

Determination of miRNA-199a and its Target Genes in

ABSTRACT :

AIM: To determine the expression profile of miRNA-199a-5p in intervertebral disc degeneration (IDD) and its correlation to the grade
of IDD.
MATERIAL and METHODS: This case-controlled study was conducted during a 6-month period from 2017 to 2018 in two university
hospitals in Shiraz, Iran. We included 15 patients with grade 3 and 4 of Pfirrmann and 5 patients with traumatic lumbosacral
fractures with grade I. Total discectomy was performed in all the individuals and the samples were sent to the laboratory. The NP
cells were isolated and the RNA was extracted. cDNA was synthesized by reverse transcriptase and the expression was measured
using real-time polymerase chain reaction (RT-PCT).
RESULTS: We overall included 20 patients in two study groups. Both study groups were comparable regarding the baseline and
clinical characteristics except for age (p=0.026). The fold change (p=0.007) and relative expression (p=0.012) of the miRNA-199a-5p
was found to be significantly higher in patients compared to controls. The fold change (p=0.001) and relative expression (p<0.001)
were also associated with the Pfirrmann grading. We found that the area under curve (AUC) was 0.880 (95%CI: 0.721-0.938)
indicative of moderate accuracy.
CONCLUSION: Expression of the miRNA-199a-5p is increased in the IDD. The expression of the miRNA-199a-5p was also
associated with the grade of the degeneration based on the Pfirrmann grading.
KEYWORDS: Intervertebral disc degeneration, MicroRNA-199a, Target genes, Pfirrmann grade

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